Evaluation of the Scrub Practitioners' List of Intraoperative Non-Technical Skills (SPLINTS) system

Peer reviewedPostprin

A Group-Based Yule Model for Bipartite Author-Paper Networks

This paper presents a novel model for author-paper networks, which is based on the assumption that authors are organized into groups and that, for each research topic, the number of papers published by a group is based on a success-breeds-success model. Collaboration between groups is modeled as random invitations from a group to an outside member. To analyze the model, a number of different metrics that can be obtained in author-paper networks were extracted. A simulation example shows that this model can effectively mimic the behavior of a real-world author-paper network, extracted from a collection of 900 journal papers in the field of complex networks.Comment: 13 pages (preprint format), 7 figure

Experience matters: comparing novice and expert ratings of non-technical skills using the NOTSS system

Abstract There is growing evidence that non-technical skills (NTS) are related to surgical outcomes and patient safety. The aim of this study was to further evaluate a behaviour rating system (NOTSS: Non-Technical Skills for Surgeons) which can be used for workplace assessment of the cognitive and social skills which are essential components of NTS. A novice group composed of consultant surgeons (n = 44) from five Scottish hospitals attended one of six experimental sessions and were trained to use the NOTSS system. They then used NOTSS to rate surgeons' behaviors in six simulated scenarios filmed in the operating room. The behaviours demonstrated in each scenario were compared to expert ratings to determine accuracy. The mode rating from the novice group (who received a short training session in behaviour assessment) was the same as the expert group in 50% of ratings. Where there was disagreement, novice raters tended to provide lower ratings than the experts. Novice raters require significant training in this emerging area of competence in order to accurately rate nontechnical skills

Strategies for improving quality and safety in global health:Lessons from nontechnical skills for surgery implementation in rwanda

The Non-Technical Skills for Surgeons (NOTSS) framework is a taxonomy of cognitive and social skills that foster expertise and medical knowledge in the operating room. This framework can be used as a method to improve the quality of surgical care in global efforts to improve access to affordable surgery

Optimised insert design for improved single-molecule imaging and quantification through CRISPR-Cas9 mediated knock-in

© 2019, The Author(s). The use of CRISPR-Cas9 genome editing to introduce endogenously expressed tags has the potential to address a number of the classical limitations of single molecule localisation microscopy. In this work we present the first systematic comparison of inserts introduced through CRISPR-knock in, with the aim of optimising this approach for single molecule imaging. We show that more highly monomeric and codon optimised variants of mEos result in improved expression at the TubA1B locus, despite the use of identical guides, homology templates, and selection strategies. We apply this approach to target the G protein-coupled receptor (GPCR) CXCR4 and show a further insert dependent effect on expression and protein function. Finally, we show that compared to over-expressed CXCR4, endogenously labelled samples allow for accurate single molecule quantification on ligand treatment. This suggests that despite the complications evident in CRISPR mediated labelling, the development of CRISPR-PALM has substantial quantitative benefits